Abstract “Listeriosis” is the recent pandemic caused by a bacterium named Listeria monocytogenes an intracellular pathogen causes very high mortality rates in the United States. In the Current study, an effort was taken to design a Potent Subunit vaccine against L. monocytogenes applying the reverse vaccinology approach. The method includes screening of complete proteome based […]
Abstract Single-nucleotide polymorphisms (SNPs) play a major role in the understanding of the genetic basis of many complex human diseases. The current study reports, of the total of 682 SNPs in the CHK2 gene, 10 non-synonymous SNPs (nsSNPs) were identified as functionally significant. SIFT, PolyPhen and I -Mutant programs were used to analyze the nsSNPs. […]
Abstract Rennet, a microbial coagulant, is obtained from Mucor miehei which is isolated from ruminant stomach. Cheese is one of the milk product which is produced from the enzyme rennet. The aim of the current work was to produce cheese from Rhizomucor miehei which is a common inhabitant of cow dung. Pure culture of the […]
Abstract Globular protein Bovine Serum Albumin is water soluble and is sensitive to Low Power mm Wave (Micro wave) irradiation leading to Coagulation at the isoelectric Point.The objective of the work was to study the effect of these radiations on the coagulation and light absorbance of purified bovine serum albumin protein. When a heat stress […]
Abstract Date rape drug refers to a drug that can be used to assist in the execution of a sexual assault, such as date rape. Sexual assault is any type of sexual activity that a person does not agree to. Drugs used to facilitate rape may have sedative, hypnotic, dissociative and/or amnesiac effects, and can […]
Abstract Butyryl cholinesterase (BCHE) is a protein belonging to ester lipase super family. It hydrolyzes choline esters in addition to its role in nerve impulse transmission. BCHE has three active sites located at 226, 353 and 456 which are responsible for acyl ester intermediate, and charge relay system. Among the three sites, 226 (serine) have […]
Abstract Over the past twenty years, DNA analysis has been revolutionalized forensic science and has become a dominant tool in law enforcement. The DNA is unique for each and every person. This application is used in forensic science to identify the criminals. The DNA is isolated from the biological samples collected from the crime scene. […]
Analysis of Variations in Conserved Motifs of Cag A protein in Different Helicobacter Pylori Strains
Abstract Infection with strains of Helicobacter pylori that carry the cytotoxin-associated antigen a (cagA) gene is associated with gastric carcinoma. Recent studies have shed light on the mechanism through which the cagA gene product, CagA, elicits pathophysiological actions. Helicobacter pylori infection has an association with histological gastritis, gastric atrophy, gastric cancer, and mucosa-associated lymphoid tissue […]
Abstract DNA fingerprinting also called DNA Typing in genetics is the method of isolating and making images of sequences of DNA (deoxyribonucleic acid). The technique was developed in 1984 by the British geneticist Alec Jeffrey’s, after he noticed the existence of certain sequences of DNA (called mini satellites) that do not contribute to the function […]
Abstract DNA evidence is being increasingly used in court cases. The first DNA typing methodology used forensically required comparatively large amounts of starting material. Now, with the use of the polymerase chain reaction (PCR) to amplify Short Tandem Repeat (STR) segments of DNA, laboratories are able to analyze DNA from samples as small as a […]