Role of Genetic Transformation in Recombinant DNA Technology and Demonstration Using Escherichia coli strain DH5alpha

Abstract In Molecular Biology, genetic transformation occurs when an organism’s character is changed or modified by inserting one or more desired gene(s) in order to utilize them for different purposes, for instance, the betterment of plant species by insertion of genes coding for drought, pest, and frost resistance, transformation of common bacterium E. coli in […]

Study on the Prevalence of Virulent genes in Escherichia coli isolated from Fish using multiplex PCR

Abstract A one shot multiplex Polymerase Chain Reaction PCR was developed for detecting 6 virulent genes of diarrheagenic E.coli from fish and shrimps. In order to differentiate between 5 categories of diarrheagenic E.coli ,I selected the target genes eae for Enteropathogenic E.coli(EPEC), ipaH for Enteroinvasive E.coli (EIEC) ,stx for Enterohaemorragic E.coli (EHEC), aggR for Enteroaggregative […]

Comparative Assessment of Antibacterial Activity of Secondary Metabolites from Three Medicinal Plants: Ocimum sanctum, Azadirachta indica and Mentha arvensis

Abstract Indiscriminate use of antibiotics has ensued into pathogen resistance, therefore, it is essential to discover the natural alternatives to combat the infectious diseases caused by these bacteria, and one scientific approach is to examine the antibacterial activities of the secondary compounds from medicinal plants that are considered to have significant prophylactic properties from centuries. […]

Studies on biochemical components, secondary metabolites and photosynthetic pigments in Mealey bug infected Terminalia arjuna-a primary host plant of Tasar Silkworm Anthereae mylitta drury

Abstract Mealey bug (Maconellicoccus hirsutus) is a serious pest of Terminalia arjuna which is the primary food for Tropical tasar silkworm Anthereae Mylitta Drury. It attacks the leaves of the host plant causing considerable damage which alters the leaf quality. An attempt was made to know the changes in the Biochemical components (Total soluble proteins, […]

Effect of Plant Growth regulators on Morphological, Physiological and Biochemical parameters of Soybean (Glycine max l. Merrill)

Abstract Plant growth and developmental processes are very much regulated by certain chemical substances called Growth regulators. Growth regulators are known to improve the physiological efficiency including photosynthetic ability and can enhance the effective partitioning of accumulates from source and sink in the field crops. The present study was conceptualized and executed with the prime […]

Production and purification of the Tyrosinase enzyme from Soil bacteria

Abstract Tyrosinases are a group of bifunctional enzymes that are involved in the hydroxylation of mono phenols to o-diphenols. These enzymes have their potential applications in several domains like Biosensors, biocatalysis in several pathways, and production of L-DOPA from tyrosine. Detection and removal of phenolic compounds from wastewater also involves the use of tyrosinases. In […]

Combining ability and gene action studies for yield and yield contributing traits in tomato (Solanum lycopersicum L.)

Abstract Eight diverse lines of tomato were crossed with three testers in line x tester mating fashion to study combining ability effects and gene action for yield and yield contributing traits. The analysis of variance revealed that the variance due to line x tester effects were highly significant for all the traits under study. The […]

Effect of integrated nitrogen management on soil available nutrients, nutrient uptake and enzyme activity of soil at harvest of safflower (Carthamus tinctorius L.)

Abstract A field experiment was conducted during rabi 2009 -10 on sandy loam soil at Student’s Farm, College of Agriculture, Rajendranagar, Acharya N.G Ranga Agricultural University, Hyderabad, A.P, India to study the effect of organic manures in combination with inorganic nitrogen fertilizer on soil available nutrients status, nutrient uptake and enzymes ( urease and dehydrogenase) […]