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Over the last few years, the undesirable impacts of fossil fuel on the environment and resulting global warming, progressive demand for energy, inevitable reduction of the world‘s energy supply, and the unstable oil market (such as the energy crisis of the 1970s) have renewed the interest of society in searching for substitute fuels. Ethanol as a neat fuel or uniform in the blended form with gasoline has a long history as automotive fuel. Microorganisms play an important role in production of ethanol from renewable resources and thus, selection of suitable strain is essential for separate process. Adding onto this for the production of bioethanol microorganisms play a key role and till date number of species are being used for the process to be carried but still there are some of the species that are yet to be explored, one of this comes under the phylum Basidiomycota as Coprinus cinereus. for the higher production of bioethanol gene silencing technology was used so as to focus onto one of desired pathway for the project since once RNA interference was applied the microbe was subjected to the only pathway which was required and the utilization of carbohydrate in some other pathway was blocked.
At the beginning of the 20th century, many industrial materials such as dyes, solvents, and synthetic fibers were prepared from trees and agricultural crops. By the late 1960s, however, many of these bio-based chemical products were swapped by petroleum byproducts which could be produced at lower cost [1, 2]. Bioethanol is being widely investigated as a renewable fuel source because in several respects it is superior to gasoline fuel. Ethanol delivers energy that is renewable and less carbon concentrated than oil . Production of fuel ethanol from biomass seem like to be an interesting alternative to traditional fossil fuel, which can be applied as a sole fuel in cars with loyal engines or in fuel blends. Ethanol is presently produced from sugars, starches and cellulosic materials. Cellulosic resources obtained from wood and agricultural residuals, municipal solid wastes and energy crops signify the most abundant global source of biomass  . Ethanol as a neat fuel or uniform in the blended form with gasoline has a long history as automotive fuel.
On a global scale, synthetic ethanol accounts for about 3-4% of entire production while the rest is produced from fermentation of biomass – mainly sugar crops, e. g. cane and beet, and of grains (mainly corn) . Ethanol as a neat fuel or uniform in the blended form with gasoline has a long history as automotive fuel.
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