Targeting of Leptin Protein to Treat the Complications in Obesity, An Insilico Drug Designning

Author Name: *Biswajit Satapathy, Pritam Kumar Panda, Suman Mishra, Samaya Chakra Pradhan, Subhashree Hota, Piyus Pritam
Author Email: biswasat92@gmail.com

Abstract

The objective of the work is to analyze the diabetic protein Leptin, a major cause of several syndromes in the current era. The work involves the characterization of the Leptin protein and to establish its relation in Obesity. The protein is targeted for the treatement of obesity symptoms using several chemical ligands. The work included Drug Designing towards the obesity syndrome considering leptin as a receptor. The chemical compounds were collected from several data bases like ChEBI, NCBI etc. The collected chemicals were screened and the best compounds having good drug likeliness, non toxic and lowest ambiguity were finalized to dock with the selected receptor. The compounds were docked using the HEX software. The energy evaluation was performed and the best chemicals were selected to dock with the LEPTIN protein. Based on the docking results the best binding affinities were screened and the best drug was proposed.

Introduction

Anti obesity diet and medications are the hot cakes in the market due to the ever increasing problem of obesity. The major reason for the outbreak of this problem is the sedentary life style of man. There is also a genetic factor that causes obesity in the normal individuals. The obese (ob) gene, also called as th leptin gene, the mutation of which results in severe hereditary obesity and diabetes in mice. It has recently been isolated through positional cloning. The nucleotide sequences of the human ob cDNA coding region were 83% identical to those of the mouse and rat ob cDNA coding regions. Analysis of the deduced amino acid sequences revealed that the human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Leptin is one of the genetic causes involved in Obesity and othger diet related disorders. It is a hunger related gene that causes the feeling of hunger and maintains the diet. The improper balance in this gene expression or mutations would be one of the causes of diabetes. LEPTIN (ACCESSION NO: NP_061764) NORMAL FUNCTION OF GENE:

Conclusion

Obesity is one of the common syndromes seen in the current era. The rate of epidemic is increasing day by day due to the sedentary life style. It is the high time to fight against the problem and eradicate the same. The current work aims to design a drug against the symptoms of Obesity using Leptin protein as the target receptor. Leptin is also known as obase protein due to its major role in diet and body mass. This is a protein that signals for hunger. Literature study reveals that the mutation at the protein and molecular level would lead to obesity complication. Thus, the current work involves targeting of the Leptin receptor with the suitable chemicals that has the property to block the effect of leptin mutations. The Chemicals were collected from the PubChem database and were subjected for intensive screening for the Druglikeliness, Toxicity and Ambiguity. The best chemicals qualifying the required parameters were selected to dock with the same receptor. The energy evaluation was performed and the best chemicals were selected based on the lowest energy and highest stability. The final chemical selected to target leptin was Fluoxetine Hydrochloride with the energy -226.64

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