Antigenic Epitope Prediction towards SARS Corona Virus using Bioinformatics Analysis

Author Name: *Sunitha Panigrahi,Nikkitha Umesh,
Author Email: sritha17@yahoo.com

Abstract

Severe Accute Respiratory Syndrome is one of the types of Pneumonia, wherein the Victim suffers from severe Breathing Problem. It is a viral infection where in the Organism responsible is SARS Co V. In worst condition the patient may even die. Due to its intense effect there is a huge demand to develop a treatment methodology to cure and save the lives of the people. The best approach to solve the problem is pretension rather than cure. To prevent this dreadful disease development of the Vaccine is the only approach possible. Here the research involves the use of Computer aided reverse vaccinology approach to identify and design the most potent antigenic peptide, the destruction of which can cure the disease. More than 100 proteins of the Virus have been screened from the Public database NCBI. The proteins that are confirmed to exist are further selected. The selected proteins are compared against the human proteome using BLAST analysis to eliminate the proteins that are common among the two species. The selected proteins are further analyzed to identify the antigenic regions upon it that can actually cause the effect of pathogen. The selected peptides are further compared to identify the most effective peptide that has maximum pathogenecity. The peptide is finally designed and optimized to calculate the energy using Argus lab software.

KeyWords

SARS Co V, Antigenic Epitope, Reverse Vaccinology, BLAST

Introduction

A new and deadly clinical syndrome now called severe acute respiratory syndrome (SARS) was brought to the attention of the WHO by Dr. Carlo urbani and his colleagues in a Vietnamese hospital in February 2003[1]. WHO, the medical staffs in hospitals where the disease had appeared, and local and regional governments, together with a dozen cooperating laboratories across the globe, immediately responded. They provided a pro-visional case definition to identify the extent and geo-graphic distribution of the outbreak [2] the infectious agent, and travel advisories and quarantines to limit the spread of the disease [3] (CoV), designated SARS-CoV was quickly identified as the etiological agent [4, 5] have not established, evidence suggests that it is an animal virus that was recently transmitted to humans [6] wildlife species consumed as delicacies in southern China, including Himalayan masked palm civets, Chinese ferret badgers, and raccoon dogs, possess antibodies consistent with natural infection with related CoVs, laboratory investigations to identify .Although the origin of the virus. A novel corona virus.

Conclusion

The current work aimed to develop or identify a potential vaccine candidate (protein) against the SARS Corona Virus causing SARS Disease. The proteins of the organism are collected from the Public database NCBI followed by their comparison to human proteome using BLAST for identification of antigenic proteins. Later the antigenic proteins were further analyzed for the identification of Antigenic Propensity and antigenic region on the proteins. Based on the results one of the peptide is finalized and selected. The selected peptide was FKDNVILLNKHIDAY which was commonly present in several nucleocapsid proteins of the virus especially present in the dimerization region. The peptide was constructed in Argus lab and the energy was calculated to be 181.47Kcal/mol. The peptide can be artificially synthesized and attenuated so as to make it a potential vaccine to prevent SARS. This work lays a foundation for then development of the selected peptide vaccine.

9 total views, 0 views today

Download File

About the author: dev