Effect of Rhizodyne on growth patterns of local cultivars of Paddy (oryza sativa L.) using paper towel and pot culture assay methods

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Abstract

In the current Study pot assay has been employed to investigate the effect of commercial biofertilizer (Rhizodyne – complex bio fertilizer) application on 4 rice local cultivars (oryza sativa L.) growth both shoots and roots in local lake soil. Rhizodyne is a product of Varsha Biosciences and Technology (VBTIPL), Hyderabad (India) that contains species of Phosphate solubilising bacteria(PSB), Azospirillum species, K mobilizing bacteria and Zn solubulising bacteria (5 * 107 CFU minimum per gram of rhizodyne). In paper towel experiment of studied parameters in 4 cultivars of Indica rice Cotton dora sannalu response was maximum in shoot length, root length and weight of root and in weight of shoot Erramallelu showed maximum weight. In seeds treated with rhizodyne of studied parameters in 4 cultivars of Indica rice Cotton dora sannalu response was maximum in shoot length, root length and weight of root and in weight of shoot Erramallelu showed maximum weight, No of tillers were more in Pothana. In soil treated with rhizodyne of studied parameters in 4 cultivars of Indica rice Erramallelu was maximum in shoot length, root length and weight of shoot and in weight of root Cotton dora sannalu showed maximum weight, No of tillers were more in Pothana

Introduction

Rice (Oryza sativa L.) is probably the most important cereal in the world and serves as food for about 50% of the world’s population (Ladha et al., 1997). This crop requires about 10 million tons of N fertilizers every year worldwide (IRRI, 1996). Rice is usually grown in N-deficient soils, and this element must be supplied to the field by commercially available N fertilizers. However, a substantial amount of urea-N or NO3-N applied as fertilizers is lost through different mechanisms, thus causing environmental pollution problems. Use of biological N2-fixation
technology can decrease N fertilizer application and reduce environmental risks (Raimam et al.,
2007). This process can contribute as much as 75 kg N ha-1 per crop cycle with means of 8 to 30 kg N ha-1(Irissarri and Reinhold-Hurek, 2001). These N-fixing bacteria may be free-living or naturally associated to rice plants. Recent studies have shown that several bacteria
may be isolated from sterilized surface roots of flooded rice plants, suggesting endophytic colonization (Raimam et al., 2007). The most likely candidates for biological N fixation in rice are species of Alcaligenes, Azospirillum, Bacillus, Enterobacter, Herbaspirillum, Klebsiella, Pseudomonas, and Rhizobium (Malik et al., 1997; James et al., 1999; Yanni et al., 1997). After laboratory testing and studying field performance, efficient bacterial strains for biofertilizer production were finally selected. A suitable carrier material was selected after trying a number of local materials to ensure maximum survival of the inoculated bacteria during storage and transportation. They named this biofertilizer as Rhizodyne, which improves crop growth and yield by increasing photosynthesis, producing bioactive substances, such as hormones and enzymes, controlling soil diseases, and accelerating decomposition of lignin materials in the soil (Higa, 2000; Hussain et al., 2002).

Conclusion

Rhizodyne has a positive effect on shoot length root length, no of tillers and weights of shoots and roots of studied rice cultivars in paper towel assay, seeds treated with rhizodyne and soil treated with rhizodyne when compared with control.

Acknowledgements

Authors are grateful to CII (confederation of Indian industry), DST (Department of Science and Technology, Ministry of Science & Technology, New Delhi, India), Varsha Bioscience and Technology Private Limited for providing the Prime Minister’s Fellowship (S. Reddy). Similarly, Shri Neeraj Verma, Director In-charge (State Farms Corporation of India Limited) for authenticating and providing 6 Indica rice cultivars and Dr. C. Cheralu, Associate Director Agricultural Research Station, Warangal, India for authenticating and providing 15 Indica rice cultivars for our present study.

References

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