Comparative estimation of Penicillin production by wild and UV irradiated mutant strains of Penicillium chrysogenum

Author Name: Kotte Karunakar1, Naveen Veeragani, Jyothsna Gundlapally, Triveni Gummadi
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Penicillin is one of the well known antibiotics that are produced by Penicillium species of Fungi. Due to the immense applicability of penicillin antibiotic in the field of medicine it has gained a very vital position in the industrial sector. It is used to treat several bacterial infections due to its innate antibacterial activity. In view of the importance of penicillin in the field of industry and medicine the current work aims to standardize the effects of mutations on the Penicillium productivity by P.chrysogenum. The strain chrysogenum was selected as it is the well known organism commercially used for Penicillin production. The work involves the isolation of P.chrysogenum culture from the contaminated papaya followed by its pure culture preparation. The mutant strains were prepared by subjecting the wild strains to UV irradiation for various time durations. All the strains were inoculated into the Production medium respectively. The antibiotic produced was estimated using titrimetric protocol and the comparative study was done on the productivity of all the treated cultures. The work concludes that the effect of U.V mutations on the productivity of the Penicillin by P.chrysogenum depends upon the exposure time. The productivity increases regularly for a certain period of exposure time after which it starts decreasing. This can be a very useful method for the industrialist to analyze and standardize the specific mutational conditions which can produce high yield of the product with minimum source.


Wild, Mutant, UV irradiation, Penicillium chrysogenum, Penicillin


The “Wonder Drug” Penicillin is an antibiotic produced from Penicillium chrysogenum [1]. Antibiotics are created as part of the life process of one organism, which can kill or stop the growth of a disease-causing microbe [2]. Alexander Fleming was the first person to propose the concept of Antibiotics. Penicillin is the antibiotic produced by several species of Penicillium which has the capability to inhibit the growth of bacteria around its colony [3].


From the results of quantitative estimation and qualitative efficacy study it can be concluded that the treatment with UV irradiation has a direct influence on the penicillin producing ability of the fungi. The effect of this radiation is dependant upon the exposure time in a variable manner. Initially the rate of Production of penicillin increases with the duration of exposure directly up to a certain limit. Later suddenly the rate of penicillin production decreases with the increased exposure time. From the above work it can be stated that the industrialist should initially analyze the optimum exposure time for the maximum penicillin production to obtain high yield from the stock cultures

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