Transformation of Penicillin Resistance Plasmid from Penicillin Resistant soil Bacteria to E.coli DH5α

Abstract Transformation, an active uptake of free DNA by bacterial cells and the heritable incorporation of its genetic information is one of the most adventurous mechanism that can be manipulated with the technology to produce genetically modified and manipulated organisms that can fulfil the needs of the user.. Only more recently has it been considered […]

DNA Paternity Testing: An Analysis of Forensic Aspects

Abstract DNA testing is currently the most advanced and accurate technology to determine paternity. It has come a long way from application in immigration case to identification of foetus of rape victims and missing persons/ deceased. DNA paternity testing in forensic casework is greatly based on comparison with reference biological samples of close blood relatives. […]

Biochemical Responses of Cicer arietinum Seedlings to Zinc And Bioengineered Zinc Nanoparticles Stress

Abstract The effect of bioengineered zinc nanoparticles and zinc on biochemical analysis was investigated on the Cicer arietinum Seedlings. The extracellular synthesis of zinc nanoparticles with an average size of 47.6 nm was characterized by Transmission electron microscopy. Heavy metals are important and essential components in plant growth. However, when they increase in concentration, heavy […]

Development of Multiplex PCR for Rapid Detection of Toxigenic Strains of Staphylococcus aureus from Food Samples

Abstract This study developed a multiplex PCR method for the screening and detection of five toxigenic strains of Staphylococcus aureus. The multiplex PCR procedure, which uses five pairs of primers produced specific amplicon of expected sized of SEA, SEB, SEC, SED and SEE genes which are responsible for producing Enterotoxins. These are the target genes […]

Identification of Vaccine targets in Klebsiella pneumonia using Violin

Abstract Klebsiella pneumoniae causes community acquired, nosocomial infections & other diseases like bacteremia, hepatic abscess, meningitis, respiratory & urinary tract infections worldwide. This organism is rod shaped, gram-negative, non-motile encapsulated, lactose fermenting facultative anaerobic bacterium, found as the normal flora on the mouth, skin and intestine. Klebsiella pneumoniae belonging to the Enterobacterceae family typically expresses […]

Efficacy of talc-based formulation of Pseudomonas fluorescens on the management of leaf spot disease of Stevia rebaudiana Bertoni

Abstract Stevia rebaudiana Bertoni, a natural sweetener contains two main sweetest compounds, stevioside (ST) and rebaudioside A (R-A), tasting about 300 and 450 times sweeter than sucrose, respectively. This commercially important plant also suffers a leaf spot disease caused by the fungus Alternaria alternata in various districts of Tamil Nadu, India. In the present study, […]

Efficacy of some natural flavanoids against PDK-1 Kinase: An insilico Molecular docking and Molecular Dynamic Simulation study

Abstract PDK-1, 63Da serine thionine kinase has a well-known role in cell growth regulation and differentiation. It is over expressed in many cancer cells and considered as a novel target of many anti-cancer drugs. In the present study we have explored the affinity and interaction of some plant derived natural flavanoids against PDK-1 with insilico […]