Morpho functional Parameters of Blood Lymphocytes, in the Process of Adaptation of Chickens to Lighting Regimes

Author Name(s): Lyudmila K. Buslovskaya, Aleksey Yu. Kovtunenko, Yulia P. Ryzhkova, Svetlana D. Chernyavskikh, Elena V. Golovko
Author Email: buslovskaya@bsu.edu.ru

Abstract

The dynamics of morphometric and functional parameters of blood lymphocytes of chickens, in the process of adaptation to lighting regimes, was studied using the atomic force microscopy. It was established, that in the process of adaptation, the initial in duration of cells was replaced by an increase in their volume and surface area, the modulus of elasticity and the surface potential of lymphocyte membrane under these conditions had decreased, increasing the liquid properties of membrane, thereby providing cellular mechanisms of adaptation.

Introduction

Lymphocytes are the heterogeneous population, whose elements differ from each other in origin, structure, functions and morphological characteristics. These cells accumulate, store and transfer information about adaptive and disadaptive processes in the body, participating in the physiological mechanisms of life-sustaining activity. It is known, that the morphometric parameters of blood cells are highly sensitive and objective criteria for assessing homeostatic disorders [1-4]. The method of atomic force microscopy makes it possible to study morphofunctional changes, occurring at the level of lymphocytes [5, 6]. This allows their use when analyzing the adaptive reactions of the organism and assessing the stress reaction.

Conclusions

  1. In the process of chickens’ adaptation to different lighting regimes, there are significant changes in the morphofunctional parameters of blood lymphocytes.
  2. During the adaptation of chickens to lighting regimes, the staging is observed in the change of morphometric parameters of blood lymphocytes, the compaction is replaced by an increase in their volume and surface area that indicates the change in the functional state of cells.
  3. Surface potential and modulus of elasticity of the chicken blood lymphocyte membrane, in the process of adaptation to lighting regimes decrease, increasing its fluidity and permeability, thereby ensuring the activity, immune properties of cells and cellular mechanisms of adaptation.

References

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