Post Mortem Analysis of General Changes in Viscera

Author Name: *Madhurima Kalsekar, O Sreeharshini
Author Email: madhurima.kalsekar@gmail.com

Abstract

Postmortem changes begin soon after death and progress along a timeline. This study presents an analysis on the changes in the physical properties of goat organs and change in its DNA content after its death. Four organs of goat i.e liver, kidney, lungs and intestine were considered as working samples. The samples were cut into two halves so that one can be used for the study of general chenges and the other can be utilized for DNA extraction at regular intervals of time. The techniques like Agarose gel electrophoresis and UV spectroscopy was performed to check the presence of DNA and to quantify the extracted DNA samples. The same protocol was performed for a period of 5 days and in the end agarose gel electrophoresis was performed to detect the presence of DNA, bands were observed confirming the quality of the DNA. Weight changes and changes in the color were also observed and recorded. This analysis can be useful to analyze the age of the dead bodies and the further investigative protocols.

Keywords

Post-mortem changes, goat organs, physical properties, Agarose gel electrophoresis, UV Spectroscopy

Introduction

A post-mortem analysis is the examination of a body after death and can also be called an autopsy. The interval between death and the time of examination of body is called post-mortem interval. This analysis is very crucial to know when the crime was committed. The most significant medico- legal issue in any post-mortem examination is to find out time since death. [1] One of the most crucial longstanding problems in the field of forensic medicine is the determination of the time of death upon the detection of a possible homicide victim [2]. Postmortem changes begin soon after death and grows along a timeline. Two processes, putrefaction and autolysis, begin to transform the body; either one may dominate, depending on the situations surrounding death, as well as the climate. Putrefaction involves the action of bacteria on the tissues of the body. This process, ubiquitous in moist climates, is associated with green discoloration of the body; gas production with bloating combined; skin slippage; and a bad odor. Autolysis is the disruption of the body by endogenous substances. It proceeds most speedily in organs such as the pancreas and stomach. It may predominate in more arid conditions and can ultimately result in mummification. In most conditions, autolysis and putrefaction occur in tandem. In temperate climatic conditions, they can proceed in rapid degradation of the tissues. These alterations may eventually produce great distortion of the body after death.[3]

 

Conclusion

It has been a major problem in the field of forensic science to find out the post-mortem time interval i.e time since death. PMI can be a very important factor to find out the criminal in case of murder. It gives an initiative to the police to start the investigation. The current work involves the study of various tissues after its death of the tissue. At times when no other method can be used to find out the time since death this approach can be a useful method. The determination of the quantity of DNA should be an objective and exact way to estimate the PMI.

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