Isolation and Identification of Pseudomonas aeruginosa from different sources (soil, wound, urine) and Checking its MIC with various Antibiotics

Author Name: *Hayder Abdulrahman majeed, Raed Obaid Saleh AL-Sabhany
Author Email: *Hayderabd1219@gmail.com

Abstract

Pseudomonas is a group of bacteria found in soil, water, skin flora and most man- made environments throughout the world. Pseudomonas infections may cause disease, generalized inflammation and sepsis. The targeted organism for this organism are the lungs, the urinary tract, and kidneys, the result can be fatal. It can also be found on medical equipment.  Pseudomonas is well known to be animal and plant pathogen. It is also known to cause food spoilage. Over 50 different types of pseudomonas can infect humans, but most infections are caused by pseudomonas aeruginosa, which is known for its role as an opportunistic human pathogen. In the present study a total of 10 soil samples, 25 samples of urine and 25 samples of wound were collected from Health centre, Osmania University for the isolation of pseudomonas spp. All the samples were inoculated onto selective medium “Kings Media” for isolation of all pseudomonas spp. and Cetrimide agar for isolation of pseudomonas aeruginosa.  A total of three pseudomonas aeruginosa isolates were selected for further studies taking one pseudomonas aeruginosa. from each of the source. All the three were identified by biochemical characteristics. For all the three strains Antibiotic susceptibility testing (AST) was carried out to determine the antibiotic sensitivity and resistant in plate culture conditions. Presence of plasmid DNA indicated the possibility of antibiotic resistance in pseudomonas spp. and also MIC (Minimum Inhibitory Concentration) for three strain of pseudomonas aeruginosa was detected.

Keywords

Pseudomonas, Cetrimide agar, Kings Media, MIC, Opportunistic Pathogen.

Introduction

Pseudomonas is a genus of gram-negative, aerobic gammaproteobacteria, belonging to the family Pseudomonadaceae containing 191 validly described species [1] The members of the genus demonstrate a great deal of metabolic diversity, and consequently are able to colonize a wide range of niches [2] Their ease of culture in vitro and availability of an increasing number of Pseudomonas strain genome sequences has made the genus an excellent focus for scientific research; the best studied species include P. aeruginosa  in its role as an opportunistic human pathogen, the plant

Pathogen P. syringae, the soil bacterium P. putida , and the plant growth-promoting P. fluorescens. Because of their widespread occurrence in water and plant seeds such as dicots, the Pseudomonads were observed early in the history of microbiology. The generic name Pseudomonas created for these organisms was defined in rather vague terms by Walter Migula in 1894 and 1900 as a genus of Gram-negative, rod-shaped and polar-flagellated bacteria with some sporulating species [3, 4] the latter statement was proved incorrect and was due to refractive granules of reserve materials. Despite the vague description, the type species, Pseudomonas pyocyanea (basonym of Pseudomonas aeruginosa), proved the best descriptor [5

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