Since DNA is the blue print for life, every living creature contains DNA. Isolation of DNA is one of the most basic techniques in the molecular study. The standard method of DNA extraction requires several chemicals for various functions. The major chemicals used include SDS or alternative detergent for cell wall break down, ethanol for Purification, Iso propyl alcohol for precipitation, chloroform for decolouration etc. However the chemicals can also be substituted with the use of routinely available components like Regular detergent for cell wall lysis, Fruit juices for Lysozyme activity, alcohol for Precipitation etc. The aim of the current work is to use the conventional non-scientific method of the DNA extraction and to analyse its accuracy and efficiency.
This is a crude but effective method of extracting DNA from green peas. Here the tissue is broken up mechanically. Pineapple juice contains enzymes that break down proteins. The liquid detergent is used to degrade both the cell membranes those surrounding the nuclei. After the extraction the purification can also performed in a small scale. This conventional method can be compared with the standard method in terms of purity and concentration of the DNA obtained.
DNA is basically a long molecule that contains coded instructions for the cells. DNA is biologically essential for life. DNA is found in all the living organisms in every cell and it contains the storage of complex information about our nature, behaviour etc. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them. In general, the aim is to separate DNA present in the nucleus of the cell from other cellular components. DNA is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms (with the exception of RNA viruses). The main role of DNA molecules is the long-term storage of information. The DNA sequences used in the construction of recombinant DNA molecules can originate from any species. For example, plant DNA may be joined to bacterial DNA, or human DNA may be joined with fungal DNA. In addition, DNA sequences that do not occur anywhere in nature may be created by the chemical synthesis of DNA, and incorporated into recombinant molecules. Using recombinant DNA technology and synthetic DNA, literally any DNA sequence may be created and introduced into any of a very wide range of living organisms.
The modern method of DNA extraction requires several chemicals for various purposes. The current work aimed to analyse the efficacy of the use of routinely available household substances for the same purpose. The results of the study revealed that this technique of DNA extraction has yielded a good concentration of DNA with an optimum purity, thus the product can be used for further analysis. This method can be applicable under circumstances leading to the lack of proper chemicals and equipment. The method is also economic compared to the laboratory process.
- Watson, J.D.&Crick, F.H.C. The structure of DNA. Cold spring Harbor Symp. Quant. Biol. 18,123-131(1953).
- Watson, J.D. 1968. The Double Helix. Athenaeum press, New York. Reprinted in Stent, G. S., ed. The Double Helix: Text Commentary, Reviews, Original Papers (Norton, W.W., New York, 1980).
- William W. Wilfinger, Karol Mackey, and Piotr Chomczynski, Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity: BioTechniques 22:474-481 (March 1997)
- Leninger, A. L. Biochemistry, 2nd ed., Worth Publishers, New York, 1975
- Cell biology and genetics. B.D Singh, Pratibha Nallari, P.B Kavi kishor, P.Hanumantha Rao.
- Dahm, R (January Discovering DNA: Friedrich miescher and the early years of nucleic acid research.”HUMAN GENETICS 122(6):565-81
- Sambrook, Michael R. Green, Joseph. MOLECULAR CLONING. (4th ed. ed.). Cold Spring Harbor Laboratory Pr. ISBN 1936113422
- Helena Curtis, N. Sue Barnes; “BIOLOGY”,Worth Publishers,Inc., New York;
- S.C. Tan and B.C.Yiap, “DNA, RNA, Protein Extraction: the past and the present,”JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 2009, Article ID 574398, 10 pages, 2009.
- N. Do and R.P. Adams, “A simple technique for removing plant polysaccharides contaminants from DNA,”BIO TECHNIQUES, vol. 10, no. 2, pp. 162-166,1991.
DOWNLOAD THE COMPLETE RESEARCH PAPER PDF
6 total views, 0 views today